Fig. 1. Myriocin induces autophagy in CF cells. CF cells and healthy cells, treated with Myriocin for 12 hours. Detection of LC3 I and II protein expression by western blot in absence or in presence of 1 hour treatment with chloroquine and normalized on β-actin (A). Quantification of LC3II/I ratio obtained from triplicate samples and normalized onto β Actin and represented in the bars graph. Quantification of P62 protein expression by western blot analysis on whole lysates and normalized on β-actin (B); two‐tailed unpaired Student's T‐test.